SciMed
BOD Incubator 171L — 5°C to 50°C ±0.5°C, Dual Door, EPA Wastewater Testing Ready
BOD Incubator 171L — 5°C to 50°C ±0.5°C, Dual Door, EPA Wastewater Testing Ready
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171-liter (6.1 cu.ft.) Biochemical Oxygen Demand incubator engineered to the 20°C ±1°C stability target required by EPA Method 5210B, Standard Methods for the Examination of Water & Wastewater 5210, and ASTM D888. 5-50°C operating range with Deluxe Digital PID Controller holding ±0.5°C accuracy. Dual-door design with transparent acrylic inner door for observation without cold-loss. LED interior with door switch. For municipal wastewater plants, environmental testing labs, pharmaceutical stability chambers, microbiology, and academic environmental engineering programs. $14,999 direct — versus $22,000-$35,000 for Thermo Scientific / VWR / Sheldon Manufacturing (SHEL LAB) equivalents.
What a BOD incubator does
Biochemical Oxygen Demand (BOD) is the fundamental measurement of organic pollution in water. EPA Method 5210B requires wastewater samples to be incubated at 20°C ±1°C for 5 days while measuring dissolved oxygen depletion — the more DO consumed by microorganisms, the higher the BOD, the higher the organic pollution load. This measurement drives NPDES permit compliance for every municipal wastewater treatment plant and industrial discharger in the United States. The BOD incubator is the temperature-controlled environment where those 5-day incubations happen, typically running dozens of 300 mL BOD bottles simultaneously at rigidly held 20°C.
Beyond BOD, the same 5-50°C range enables microbiology (30°C, 37°C), plant tissue culture (25°C), seed germination testing, entomology rearing, pharmaceutical stability (25°C ± 2°C / 60% RH constant), and any application requiring tight temperature control across a moderate range.
Specifications
| Chamber capacity | 171 liters / 6.1 cu.ft. |
|---|---|
| Internal dimensions | 505 × 415 × 830 mm (W × D × H) |
| Temperature range | 5°C to 50°C |
| Stability | ±0.5°C (typical, at 20°C setpoint after equilibration) |
| Uniformity | ±1.0°C across chamber at 20°C |
| Controller | Deluxe Digital PID controller with LED display |
| Ambient operating | 15-32°C recommended |
| Chamber material | Anodized aluminum or highly polished SS 304 (specify at order) |
| Exterior | Thick mild steel, powder-coated matte finish |
| Outer door | Double-wall with magnetic gasket + lock & key |
| Inner door | Transparent acrylic — observe samples without cold-loss |
| Shelves | 2 or 3 adjustable perforated stainless steel shelves (specify) |
| Interior lighting | LED, door-switch activated (auto on/off) |
| Refrigeration | Sealed system, high-efficiency compressor, environmentally-friendly refrigerant |
| Heating | Integrated element for setpoints above ambient |
| Air circulation | Advanced forced-air system for uniform distribution |
| Safety | Compressor protection, over-temperature cut-out, low-refrigerant alarm |
| Power | 110V or 220V AC, 50/60 Hz single-phase (specify at order) |
| Warranty | 2-year parts + labor · 5-year compressor |
EPA Method 5210B compliance
Method 5210B (5-day BOD test) requires the incubation environment to be 20°C ± 1°C for 5 days without variation greater than 1°C in any direction. Our ±0.5°C stability at 20°C setpoint provides a 2× safety margin against the ±1°C compliance requirement — critical for NPDES permit-compliant lab data.
Method 5210B, Standard Methods 5210, ASTM D888, and 40 CFR 136 discharge monitoring requirements are all satisfied by any BOD incubator holding 20°C ± 1°C. Our controller documentation + independent verification records (from your calibration provider) provide the audit trail wastewater plants need for state / EPA discharge monitoring reports.
Applications
| Application | Setpoint | Typical use |
|---|---|---|
| EPA 5210B BOD testing | 20°C ± 1°C | Municipal wastewater plant NPDES compliance, industrial discharger monitoring |
| Microbiology — E. coli / coliforms | 35-37°C | Water quality, food safety, clinical microbiology screening |
| Microbiology — general bacteria | 25-30°C | Environmental isolates, soil microbiology, general TC |
| Pharmaceutical stability | 25°C or 30°C or 40°C | ICH Q1A(R2) intermediate + accelerated stability studies |
| Plant tissue culture | 22-25°C | Micropropagation, callus culture, agricultural biotech |
| Seed germination testing | 15-30°C | Agricultural seed labs (AOSA compliance), university programs |
| Entomology rearing | 25-28°C | Insect life-cycle studies, pesticide efficacy, biocontrol research |
| Water quality — nitrification / denitrification | 10-30°C | Nutrient removal process studies |
| Cold storage / refrigerated culture | 4-10°C | Slow bacterial growth, food shelf-life studies |
Comparison to alternatives
| Feature | SciMed BOD Incubator | Thermo Precision 815 | VWR 2005 | SHEL LAB SRI20 |
|---|---|---|---|---|
| Price | $14,999 | $22,000-$28,000 | $18,000-$25,000 | $16,000-$22,000 |
| Chamber size | 171 L / 6.1 cu.ft. | 170 L | 170 L | 170 L |
| Temperature range | 5-50°C | 5-40°C | 2-45°C | 5-40°C |
| Stability | ±0.5°C | ±0.5°C | ±0.5°C | ±0.5°C |
| Controller | Deluxe Digital PID | Digital PID | Digital PID | Microprocessor PID |
| Dual door | Yes (acrylic inner) | Yes | Yes | Yes |
| EPA 5210B | Yes | Yes | Yes | Yes |
| Lead time | Same-day US ship | 8-12 weeks | 6-10 weeks | 4-8 weeks |
| Warranty | 2yr parts / 5yr compressor | 1yr | 2yr | 1yr |
Best for
- Municipal wastewater treatment plants — NPDES permit-required 5-day BOD compliance testing (small-to-medium plants: 1-10 MGD)
- Industrial discharge permit monitoring — food & beverage, pulp & paper, chemical plants, dairy, brewery + distillery
- Environmental testing laboratories — commercial contract labs running BOD + microbiology on client samples
- State + regional water quality labs — surface water monitoring, EPA + state DEQ analysis
- Pharmaceutical stability chambers — ICH Q1A(R2) 25°C accelerated + 30°C intermediate conditions
- Microbiology + clinical labs — 35°C + 37°C bacterial culture
- University environmental engineering + microbiology programs — teaching + research
- Seed testing laboratories — AOSA-certified germination testing
- Agricultural biotech / plant tissue culture — micropropagation facilities
- Contract research + testing organizations — high-volume multi-client work
Construction — built for years of continuous duty
- Double-wall construction — insulation between inner + outer walls minimizes heat exchange with lab ambient, reduces compressor duty cycle, extends equipment life
- Inner chamber: anodized aluminum (economical, corrosion-resistant) or highly polished SS 304 (recommended for microbiology + pharmaceutical stability applications where cleaning aggressively is common)
- Outer body: thick mild steel with powder-coated matte finish — hospital / lab professional appearance, cleans easily
- Dual door design: outer double-wall door with magnetic gasket + lock & key + inner transparent acrylic door for observation without breaking temperature during 5-day BOD incubations
- Shelves: 2 or 3 adjustable perforated SS shelves (specify at order) — perforation allows air circulation around samples for uniform temperature
- Sealed refrigeration system: high-efficiency compressor with built-in overload protection, thermal safety cut-out, and environmentally-friendly refrigerant (R-134a or R-600a)
- Integrated heating element: for setpoints above ambient without secondary equipment
- Forced-air circulation: internal fan drives uniform temperature distribution — critical for the ±1°C EPA requirement across a full BOD bottle rack
- LED interior lighting: door-switch activated auto on/off; LED is preferred over incandescent because it produces negligible heat that would perturb temperature control
Buying for a wastewater plant, environmental lab, or pharma facility?
SciMed supplies BOD incubators direct to municipal wastewater plants, state / regional environmental laboratories, commercial contract testing labs, pharmaceutical stability chambers, and academic environmental engineering programs.
- Volume pricing on 3+ unit orders — multi-lab pharma stability build-outs, state DEQ network procurements
- Net-30 invoicing for verified municipal wastewater plants, state agencies, universities, pharma companies
- SAM.gov + GSA compliant procurement — federal environmental labs, EPA regional labs
- Calibration + validation documentation — factory NIST-traceable temperature verification records included; on-site calibration by certified contractors on request
- IQ/OQ documentation for pharma stability chamber deployments
- International DDP shipping — India, LATAM, MENA, SE Asia, GCC, UK, EU, AU/NZ
- Freight quotes on request — chamber ships crated ~180 kg, LTL freight typical for continental US, ocean for 3+ unit international orders
- Extended service contracts — 5-year all-inclusive service available for critical municipal + pharma installations
- Equipment financing — via lab equipment lenders (24-60 month terms)
Frequently asked questions
Does this meet EPA Method 5210B requirements?
Yes. EPA 5210B requires 20°C ± 1°C. Our chamber holds ±0.5°C at 20°C setpoint — a 2× safety margin against the compliance target. The Deluxe Digital PID controller provides continuous temperature display; recording your daily setpoint verification (or connecting an external temperature datalogger) satisfies EPA + state NPDES documentation requirements. Independent third-party temperature verification / calibration by a certified provider is standard practice for regulated labs.
How many 300 mL BOD bottles fit at once?
Approximately 40-60 standard 300 mL BOD bottles per full chamber load with 2 shelves. Bottles can be arranged in single or double rows depending on your bottle rack. For high-volume plants running 40-50 samples per week, this capacity supports full weekly workflow without batching.
Can I use this for pharmaceutical stability studies?
Yes. 25°C ± 2°C (intermediate) and 40°C ± 2°C (accelerated) conditions per ICH Q1A(R2) are within range. For 25°C / 60% RH combination testing, this is a temperature-only chamber — humidity control not included. For temperature + humidity stability, contact us for our TH-series stability chambers.
What refrigerant does this use?
R-134a or R-600a (environmentally-friendly, non-CFC, low GWP). Compliant with EPA Section 608 refrigerant regulations for US operation. No ozone-depleting refrigerants used.
How long does it take to reach setpoint?
Cool-down from ambient (25°C) to 20°C setpoint: typically 20-30 minutes to enter the ±1°C compliance band. Cool-down to 5°C: 45-60 minutes. Heat-up from ambient to 37°C: 15-25 minutes. This is comparable to Thermo, VWR, and SHEL LAB units — physics of moving 171L of air through a temperature range.
Does the interior light heat the chamber?
Minimally. The LED interior lighting is door-switch activated (only on when door is open) and LED-based (produces negligible heat vs incandescent). During normal BOD incubation with door closed for 5 days, the light is off — no temperature perturbation.
What ambient conditions does this require?
15-32°C ambient recommended for reliable performance. Cooling to 5°C from a 32°C+ hot lab is possible but reduces compressor life and slows cool-down. For labs without air conditioning in summer, we recommend positioning the incubator away from direct sunlight and away from heat-generating equipment (autoclaves, ovens). Verify at least 200 mm clearance behind the chamber for ventilation.
What voltage do I need?
Specify 110V or 220V at order. US wastewater plants + laboratories typically use 110V (standard 15A circuit). International + industrial installations typically 220V. Universal 100-240V configuration available on request (+$400).
Do you provide IQ/OQ documentation for pharma?
Yes. Installation qualification (IQ) and operational qualification (OQ) protocol templates available on request for pharma stability chamber installations. Third-party validation by a certified contractor recommended for full 21 CFR Part 11 compliance.
What's the warranty?
2-year parts + labor on chamber, doors, PID controller, refrigeration system, heating element. 5-year on compressor (typical service life 15-20 years in continuous BOD duty). Cosmetic damage, damage from operation outside 15-32°C ambient, and modifications not covered. Replacement parts stocked in San Jose warehouse for 8+ years post-purchase.
How is this shipped internationally?
US: LTL freight (crated ~180 kg, lift-gate required). International: ocean freight DDP for orders 2+ units, DHL Express DDP for single-unit urgent. Ships to India, LATAM (Mexico/Brazil/Colombia), MENA (UAE/Saudi/Egypt), SE Asia (Indonesia/Vietnam), Sub-Saharan Africa (Kenya/Nigeria/South Africa), GCC, UK, EU, AU/NZ. Custom duty/taxes handled under DDP where applicable.
Why buy direct from SciMed
- ~50% of Thermo Precision 815 pricing — same 5-50°C range, ±0.5°C stability, dual door, PID digital controller
- Same-day US ship — no 8-12 week manufacturer lead time (critical for wastewater plants replacing failed units)
- 2-year parts + 5-year compressor warranty — exceeds Thermo's 1-year standard
- NPDES + EPA-ready — factory temperature verification documentation for state DEQ / EPA discharge monitoring reports
- Municipal + industrial procurement — SAM.gov, GSA, Net-30, PO acceptance, tax-exempt purchasing
- Pharma-ready — IQ/OQ templates, calibration documentation for stability chamber deployments
- International DDP — environmental + pharma markets globally
- Extended service contracts — for critical municipal / pharma applications
Shipping: Continental US LTL freight (lift-gate required, ~180 kg crated). International DDP ocean freight or DHL Express. Municipal / environmental / pharma procurement: clinic@scimedstore.com.
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